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Phenotypic High-Throughput Screening and Drug Efficacy Test Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2026

Wang Chenchen, Wang Dongqiang, Zhang Haili, Zhu Guan

Abstract excerpt

An effective method to quantify parasite loads is key to evaluating anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening of drugs against Cryptosporidium parvum was previously infeasible using labor-intensive traditional assays. Here, we describe a simplified quantitative RT-PCR assay that directly uses cell lysates as templates, enabling high-throughput quantification of parasite...

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PMID: 41144213
DOI: 10.1007/978-1-0716-4824-7_18

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