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Rapid, sensitive, and visual detection of porcine rotavirus with RPA-CRISPR/Cas13

20 Aug 2025

Abstract excerpt

Porcine rotavirus (PoRV) is one of the major pathogens causing viral enteritis in piglets, posing serious threats to the pig industry and public health. Existing pathogen detection methods, such as RT-qPCR, suffer from complex procedures and strong reliance on equipment, making them difficult to meet the needs of grassroots laboratories or field detection. Therefore, in this study, a novel rapid and visual...

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PMID: 40909928
DOI: 10.3389/fmicb.2025.1617955

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