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Development and application of a novel beta-tubulin genotyping tool reveals host-specific transmission cluster in Balantioides coli

14 Aug 2025

Abstract excerpt

Balantioides coli is a zoonotic ciliated protozoan that infects humans and other mammals. Conventional and ITS-based genotyping approaches have limitations that hinder precise molecular epidemiological investigations. The objective of this study was to develop a new β-tubulin gene-based approach to enhance the detection and genotyping of B. coli. We performed single-cell isolation and whole-genome sequencing on...

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PMID: 40811511
DOI: 10.1371/journal.pntd.0013426

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