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Comparative evaluation of MALDI-ToF mass spectrometry and Sanger sequencing of the 16S, hsp65, and rpoB genes for non tuberculous mycobacteria species identification

28 Jul 2025

Abstract excerpt

Non tuberculous mycobacteria (NTM) infections are increasing globally, underscoring the critical importance of accurate species-level identification for effective clinical management. This study aimed to evaluate the use of three conserved markers in the mycobacterial family ( 16S , hsp65 , and rpoB ) for NTM identification through Sanger sequencing, comparing the results to those obtained using MALDI-ToF MS. A...

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PMID: 40792107
DOI: 10.3389/fcimb.2025.1612459

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