Article
Equine in vitro fertilization with frozen–thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes
9 Mar 2025
Abstract excerpt
We recently reported successful equine in vitro fertilization using fresh semen pre-incubated for a prolonged period (22 h) before co-culture with oocytes. In this study, we evaluated the feasibility of equine in vitro fertilization with frozen-thawed sperm and evaluated capacitation-related changes in these sperm over the pre-incubation period. Sperm selected via a commercial sperm separation device yielded...
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