Article
Characterization of Subcellular Dynamics of Sterol Methyltransferases Clarifies Defective Cell Division in smt2 smt3, a C-24 Ethyl Sterol-Deficient Mutant of Arabidopsis.
Biomolecules - 19 Jul 2024
Ohta Daisaku, Fuwa Ayaka, Yamaroku Yuka, Isobe Kazuki, Nakamoto Masatoshi, Okazawa Atsushi, Ogawa Takumi, Ebine Kazuo, Ueda Takashi, Mercier Pierre, Schaller Hubert
Abstract excerpt
An Arabidopsis sterol mutant, smt2 smt3, defective in sterolmethyltransferase2 (SMT2), exhibits severe growth abnormalities. The loss of C-24 ethyl sterols, maintaining the biosynthesis of C-24 methyl sterols and brassinosteroids, suggests specific roles of C-24 ethyl sterols. We characterized the subcellular localizations of fluorescent protein-fused sterol biosynthetic enzymes, such as SMT2-GFP, and found these...
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