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Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster.

Current microbiology - 17 Jul 2024

Bi Xiangdong, Wei Peng, Wang Qian, Duan Lijin, Dai Wei, Chen Rui, Zhang Dajuan

Abstract excerpt

In this study, the toxigenic characteristics of 14 strains of Microcystis were analyzed, and single nucleotide polymorphism (SNP) and insertion/deletion (InDel) loci in microcystin synthetase (mcy) gene clusters were screened. Based on SNP and InDel loci associated with the toxigenic characteristics, primers and TaqMan or Cycling fluorescent probes were designed to develop duplex real-time fluorescent...

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PMID: 39020143
DOI: 10.1007/s00284-024-03797-4

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