Article
An Inhibitor-Monitorable Single-Tube Duplex Quantitative Real-Time PCR Assay for the Detection of 'Candidatus Liberibacter asiaticus'.
Plant disease - 1 May 2024
Huang Weida, Zhong Zecheng, Lin Zhihua, Zhang Jinlian, Liu Jinhua, Chen Tingsu, Li Tingdong, Zhang Shiyin, Ge Shengxiang
Abstract excerpt
Huanglongbing (HLB) is a citrus infectious disease caused by 'Candidatus Liberibacter' spp. Recently, it has begun to spread rapidly worldwide, causing significant losses to the citrus industry. Early diagnosis of HLB relies on quantitative real-time PCR assays. However, the PCR inhibitors found in the nucleic acid extracted from plant materials pose challenges for PCR assays because they may result in...
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