Article
Preparation of leaf protoplasts from Populus (Populus × xiaohei T. S. Hwang et Liang) and establishment of transient expression system.
Journal of plant physiology - 1 Dec 2023
Yang Chengjun, Yu Ruiqiang, Li Jinbo, Wang Kai, Liu Guanjun
Abstract excerpt
Poplar, as a typical woody plant, is an ideal raw material for the production of lignocellulose biofuel. However, the longer life cycle is not conducive to the rapid identification of poplar genes. At present, protoplasts have been used for gene function identification and high-throughput analysis in many model plants. In this paper, a simplified and efficient protoplast isolation and transient expression system...
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