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Developing best practices for genotyping-by-sequencing analysis in the construction of linkage maps.

GigaScience - 28 Dec 2022

Taniguti Cristiane Hayumi, Taniguti Lucas Mitsuo, Amadeu Rodrigo Rampazo, Lau Jeekin, Gesteira Gabriel de Siqueira, Oliveira Thiago de Paula, Ferreira Getulio Caixeta, Pereira Guilherme da Silva, Byrne David, Mollinari Marcelo, Riera-Lizarazu Oscar, Garcia Antonio Augusto Franco

Abstract excerpt

BACKGROUND: Genotyping-by-sequencing (GBS) provides affordable methods for genotyping hundreds of individuals using millions of markers. However, this challenges bioinformatic procedures that must overcome possible artifacts such as the bias generated by polymerase chain reaction duplicates and sequencing errors. Genotyping errors lead to data that deviate from what is expected from regular meiosis. This, in...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 37889010
DOI: 10.1093/gigascience/giad092
PMCID: PMC10603770

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