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HoTDAM! An easy-to-use automated assay expands the inducible thermotolerance phenotype in Drosophila melanogaster: Heat hardening reduces motility.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology - 1 Dec 2023

Rokusek Blase, Cheku Sunayn, Rokusek Matthew, Waples Christopher J, Harshman Lawrence, Carlson Kimberly A

Abstract excerpt

To quantify heat tolerance in insects, two manual observation measures are typically implemented: the time to physiological collapse at a static noxious temperature (time to knockdown; TKD) or the temperature at which collapse occurs as temperature increases (critical thermal maximum; CTmax). Both assay modalities focus on physiological collapse, neglecting the prior behavioral processes. In this study, the...

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PMID: 37742820
DOI: 10.1016/j.cbpa.2023.111522
PMCID: PMC10593110

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