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Development and validation of a nanoplate-based digital PCR assay for absolute MPXV quantification

24 Aug 2023

Abstract excerpt

Quantification of mpox virus (MPXV) across different human body anatomical sites can provide insights about the most likely transmission routes, so methods able to release absolute and exact quantitative values of MPXV DNA are crucial. Here, we optimized a new QIAcuity digital PCR (dPCR) protocol for the detection and quantification of MPXV DNA in clinical samples and assessed the performance of the assay by...

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PMID: 37625622
DOI: 10.1016/j.jviromet.2023.114802

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