Article
Determination of Self-(In)compatibility and Inter-(In)compatibility Relationships in Citrus Using Manual Pollination, Microscopy, and S-Genotype Analyses.
Journal of visualized experiments : JoVE - 30 Jun 2023
Ahmad Muhammad Husnain, Zheng Xin, Hu Yu, Liu Huimin, Sun Yi, Wen Hao, Chai Lijun
Abstract excerpt
Citrus uses S-RNase-based self-incompatibility to reject self-pollen and, therefore, requires nearby pollinizer trees for successful pollination and fertilization. However, identifying suitable varieties to serve as pollinizers is a time-consuming process. To solve this problem, we have developed a rapid method for identifying pollination-compatible citrus cultivars that utilizes agarose gel electrophoresis and...
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