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Identification of fish spermatogenic cells through high-throughput immunofluorescence against testis with an antibody set

3 Apr 2023

Abstract excerpt

Image-based identification and quantification of different types of spermatogenic cells is of great importance, not only for reproductive studies but also for genetic breeding. Here, we have developed antibodies against spermatogenesis-related proteins in zebrafish ( Danio rerio ), including Ddx4, Piwil1, Sycp3, and Pcna, and a high-throughput method for immunofluorescence analysis of zebrafish testicular...

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PMID: 37077350
DOI: 10.3389/fendo.2023.1044318

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