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Rapid and Sensitive Detection of Fungicide-Resistant Crop Fungal Pathogens Using an Isothermal Amplification Refractory Mutation System.

Analytical chemistry - 21 Mar 2023

Song Sishuo, Ji Fan, Zeng Qingdong, Ding Xiaoteng, Lin Jiahao, Wang Xinsheng, Xu Yuanhong, Deng Ruijie, Li Jinghong

Abstract excerpt

Fungicide abuse leads to the emergence of fungicide-resistant fungal pathogens, thus posing a threat to agriculture and food safety. Here, we developed an isothermal amplification refractory mutation system (termed iARMS) allowing us to resolve genetic mutations, enabling rapid, sensitive, and potentially field-applicable detection of fungicide-resistant crop fungal pathogens. iARMS yielded a limit of detection...

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PMID: 36890667
DOI: 10.1021/acs.analchem.2c05115

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