Article
Multiplex gel-based PCR assay for the simultaneous detection of 5 genotypes of porcine astroviruses.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Mar 2023
Zhang Qiuya, Liu Qin, Opriessnig Tanja, Wen Dan, Gu Keda, Jiang Yonghou
Abstract excerpt
Porcine astrovirus (PAstV) has been associated experimentally with diarrhea in piglets, but much more knowledge is needed about this virus. PAstV has high genetic variability, and 5 genotypes have been identified, namely PAstV1-5. To obtain information on the epidemiology of PAstV, we established a multiplex PAstV PCR assay to detect and differentiate the 5 PAstV genotypes simultaneously. The assay utilized...
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