Developing an effective quality evaluation strategy of next-generation sequencing for accurate detecting non-small cell lung cancer samples with variable characteristics: a real-world clinical practice.

Abstract excerpt

PURPOSE: Next-generation sequencing (NGS) has been widely used in determining molecular profiling of non-small cell lung cancer (NSCLC). However, low-quality sequencing data may be generated with formalin-fixed and paraffin-embedded (FFPE) samples that have passed pre-sequencing quality control (QC). Therefore, quality evaluation of sequencing data is also critical for accurate tissue genotyping. Herein, we aimed...

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