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Development of a protein microarray for profiling circulating autoantibodies in human diseases

25 Aug 2022

Abstract excerpt

PURPOSE: To develop a robust microarray platform to detect thousands of serological autoantibodies (AAbs) simultaneously in different diseases. EXPERIMENTAL DESIGN: An AAbMap microarray was prepared by printing a total of 4032 purified His-tagged human proteins and peptide probes on a chemically-modified slide. The sensitivity, dynamic range, and the inter- and intra-array reproducibility of the AAb microarray...

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PMID: 36006834
DOI: 10.1002/prca.202100132

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