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Article

Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2.

Journal of virological methods - 1 Aug 2022

Yi Zewen, Ding Rui, Cao Ruibing, Sun Weidong, Sun Mingzhi, Dong Yongyi, Rehana Buriro, Bao Endong, Lv Yingjun

Abstract excerpt

Goose astrovirus (GAstV) is a novel pathogen that was discovered in 2018. It has two genotypes, GAstV-1 and GAstV-2, and both can cause visceral gout of goslings and result in significant economic losses. The present work aimed to develop a duplex TaqMan real-time quantitative reverse transcription PCR (RT-qPCR) assay to distinguish the two genotypes. MegAlign software was used to design two pairs of primers and...

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PMID: 35577125
DOI: 10.1016/j.jviromet.2022.114542

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Development of a duplex TaqMan real-time RT-PCR assay for simultaneous detection of goose astrovirus genotypes 1 and 2.PMID 35577125Read ↗

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