Article
[Selective method of isolating mutants sensitive to ultraviolet radiation from a culture of Rhodopseudomonas sphaeroides].
Mikrobiologiia - 1 Jan 2000
Rodina N E, Kameneva S V
Abstract excerpt
The method of penicillin selection used after UV-irradiation (lambda = 254 nm) allows one to select UV-sensitive mutants (uvs-mutants) of the phototrophous bacterium Rhodopseudomonas sphaeroides induced by nitrosomethylurea with an effectiveness greater by an order of magnitude. Over 30% of the uvs-mutants obtained using this method had an elevated sensitivity not only to far-UV (F-UV, lambda = 254 nm) but also...
Topics
- Genetic Techniques
- Methylnitrosourea
- Mutation
- Penicillins
- Radiation Tolerance
- Rhodobacter sphaeroides
- Selection, Genetic
- Ultraviolet Rays
