Article
Recombinant inbred lines and next-generation sequencing enable rapid identification of candidate genes involved in morphological and agronomic traits in foxtail millet.
Scientific reports - 7 Jan 2022
Fukunaga Kenji, Abe Akira, Mukainari Yohei, Komori Kaho, Tanaka Keisuke, Fujihara Akari, Yaegashi Hiroki, Kobayashi Michie, Ito Kazue, Ohsako Takanori, Kawase Makoto
Abstract excerpt
We constructed recombinant inbred lines (RILs) between a Japanese and a Taiwanese landrace of foxtail millet and employed next-generation sequencing, such as flexible ddRAD-seq and Nanopore sequencing to identify the candidate genes involved in the crop evolution of foxtail millet. We successfully constructed a linkage map using flexible ddRAD-seq with parents and RILs and detected major QTLs for each of three...
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