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Candidate sex-associated gene identification in Trachinotus ovatus (Carangidae) using an integrated SLAF-seq and bulked segregant analysis approach.

Gene - 30 Jan 2022

He Pingping, Wei Pinyuan, Ma Yuan, Hu Shenhua, Yao Jiuxiang, Jiang Xiaozhen, Xu Youhou, Zhu Peng, Wei Mingli, Jiang Weiming, Peng Jinxia

Abstract excerpt

It is difficult to distinguish the sexes of Trachinotus ovatus based on appearance, and little data about sex-determining genes are available for this species. Here, we generated 200 F2 individuals using the parents R404 and R403. DNA samples were collected from 50 individuals of each sex and aggregated into sex-specific DNA pools. Specific-locus amplified fragment sequencing was integrated with bulked segregant...

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PMID: 34687789
DOI: 10.1016/j.gene.2021.146026

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