Article
Nested PCR followed by NGS: Validation and application for HPV genotyping of Tunisian cervical samples.
PloS one - 1 Jan 2021
Ardhaoui Monia, Ennaifer Emna, De Matos Salim Anna Christina, Gomez Flávio Marcom, Laasili Thalja, Boubaker Med Samir, Guizani Ikram
Abstract excerpt
The most used methodologies for HPV genotyping in Tunisian studies are based on hybridization that are limited to a restricted number of HPV types and to a lack of specificity and sensitivity for same types. Recently, Next-Generation sequencing (NGS) technology has been efficiently used for HPV genotyping. In this work we designed and validated a sensitive genotyping method based on nested PCR followed by NGS....
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