Article
Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs.
Water science and technology : a journal of the International Association on Water Pollution Research - 1 Mar 2021
Lekshmi Manjusha, Kumar Sanath H, Rajendran Kooloth Valappil, Nayak Binaya Bhusan
Abstract excerpt
Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of...
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