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Endothelial colony forming cells generated from cryopreserved peripheral blood mononuclear cells.

Human immunology - 1 Apr 2021

Béland Stéphanie, Désy Olivier, Bouchard-Boivin François, Gama Alcino, De Serres Sacha A

Abstract excerpt

Derivation of endothelial colony forming cells (ECFCs) from peripheral blood mononuclear cells (PBMCs) is a technique that could provide access to donor endothelial cells to study donor endothelium/recipient immune cells interactions. The success rate of ECFC colony formation from cryopreserved PBMCs has not been reported. We used biobanked PBMCs and studied the yield of ECFC generation. Endothelial phenotype was...

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PMID: 33648804
DOI: 10.1016/j.humimm.2021.02.009

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