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Article

Simultaneous measurement of 1HC/N-R2's for rapid acquisition of backbone and sidechain paramagnetic relaxation enhancements (PREs) in proteins.

Journal of biomolecular NMR - 1 Mar 2021

Barnes C Ashley, Starich Mary R, Tjandra Nico, Mishra Pushpa

Abstract excerpt

Paramagnetic relaxation enhancements (PREs) are routinely used to provide long-range distance restraints for the determination of protein structures, to resolve protein dynamics, ligand-protein binding sites, and lowly populated species, using Nuclear Magnetic Resonance Spectroscopy (NMR). Here, we propose a simultaneous 1H-15 N, 1H-13C SESAME based pulse scheme for the rapid acquisition of 1HC/N-R2 relaxation...

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Identifiers

PMID: 33625630
DOI: 10.1007/s10858-021-00359-9
PMCID: PMC8096723

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