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Genome Sequencing of Sewage Detects Regionally Prevalent SARS-CoV-2 Variants.

mBio - 19 Jan 2021

Crits-Christoph Alexander, Kantor Rose S, Olm Matthew R, Whitney Oscar N, Al-Shayeb Basem, Lou Yue Clare, Flamholz Avi, Kennedy Lauren C, Greenwald Hannah, Hinkle Adrian, Hetzel Jonathan, Spitzer Sara, Koble Jeffery, Tan Asako, Hyde Fred, Schroth Gary, Kuersten Scott, Banfield Jillian F, Nelson Kara L

Abstract excerpt

Viral genome sequencing has guided our understanding of the spread and extent of genetic diversity of SARS-CoV-2 during the COVID-19 pandemic. SARS-CoV-2 viral genomes are usually sequenced from nasopharyngeal swabs of individual patients to track viral spread. Recently, RT-qPCR of municipal wastewater has been used to quantify the abundance of SARS-CoV-2 in several regions globally. However, metatranscriptomic...

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Identifiers

PMID: 33468686
DOI: 10.1128/mBio.02703-20
PMCID: PMC7845645

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