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Brucella melitensis and Brucella abortus genotyping via real-time PCR targeting 21 variable genome loci.

Journal of microbiological methods - 1 Jan 2021

Kılıç Selçuk, Çelebi Bekir, Turan Meral

Abstract excerpt

Brucella melitensis and Brucella abortus account for almost all cases of brucellosis in Turkish population. We developed a fourplex quantitative real-time PCR (qPCR) assay for the electrophoresis-free, rapid and cost-effective differentiation of B. abortus and B. melitensis from the other Brucella spp. The 4-plex species differentiation assay was combined with a qPCR assay targeting 17 different single nucleotide...

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PMID: 33333100
DOI: 10.1016/j.mimet.2020.106125

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