Article
Transcriptome Analysis of Testis from HFD-Induced Obese Rats (Rattus norvigicus) Indicated Predisposition for Male Infertility.
International journal of molecular sciences - 5 Sept 2020
El-Shehawi Ahmed M, El-Shazly Samir, Ahmed Mohamed, Alkafafy Mohamed, Sayed Samy, Farouk Samy, Alotaibi Saqer S, Elseehy Mona M
Abstract excerpt
Obesity is a worldwide life-threatening metabolic disorder, associated with various chronic diseases, including male infertility. Obesity was induced by high fat diet (HFD), and testis RNA was used for the transcriptome analysis using RNAseq via Illumina NovaSeq 6000 System and NovaSeq 6000 Kit. Gene expression level was estimated as FPKM (Fragments Per Kilobase of transcript per Million mapped reads)....
Topics
- Animals
- Biomarkers
- Diet, High-Fat
- Gene Expression
- Gene Expression Profiling
- Gene Expression Regulation
- Gene Ontology
- Genetic Predisposition to Disease
- High-Throughput Nucleotide Sequencing
- Infertility, Male
- Male
- Obesity
- RNA