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Article

Development of a multiplex qPCR-based approach for the diagnosis of Dirofilaria immitis, D. repens and Acanthocheilonema reconditum.

Parasites & vectors - 22 Jun 2020

Laidoudi Younes, Davoust Bernard, Varloud Marie, Niang El Hadji Amadou, Fenollar Florence, Mediannikov Oleg

Abstract excerpt

BACKGROUND: Dirofilaria immitis, D. repens and Acanthocheilonema reconditum are the main causative agents of zoonotic canine filariosis. METHODS: We developed a combined multiplex approach for filaria and Wolbachia detection using the 28S-based pan-filarial and 16S-based pan-Wolbachia qPCRs, respectively, involving a fast typing method of positive samples using triplex qPCR targeting A. reconditum, D. immitis and...

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Identifiers

PMID: 32571427
DOI: 10.1186/s13071-020-04185-0
PMCID: PMC7309989

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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