Article
High Diversity of Testate Amoebae (Amoebozoa, Arcellinida) Detected by HTS Analyses in a New England Fen using Newly Designed Taxon-specific Primers.
The Journal of eukaryotic microbiology - 1 Jul 2020
Ruggiero Alistaire, Grattepanche Jean-David, Weiner Agnes K M, Katz Laura A
Abstract excerpt
Testate (shell-building) amoebae, such as the Arcellinida (Amoebozoa), are useful bioindicators for climate change. Though past work has relied on morphological analyses to characterize Arcellinida diversity, genetic analyses revealed the presence of multiple cryptic species underlying morphospecies. Here, we design and deploy Arcellinida-specific primers for the SSU-rDNA gene to assess the community composition...
Topics
- DNA Primers
- DNA, Protozoan
- DNA, Ribosomal
- Evolution, Molecular
- Genetic Variation
- High-Throughput Nucleotide Sequencing
- New England
- Phylogeny
- Pilot Projects
- Sequence Analysis, DNA