Article
Genome survey and development of polymorphic microsatellite loci for Sillago sihama based on Illumina sequencing technology.
Molecular biology reports - 1 Apr 2020
Qiu Bixun, Fang Shaobin, Ikhwanuddin Mhd, Wong Lilian, Ma Hongyu
Abstract excerpt
In this study, we first conducted a genome survey assay for Sillago sihama by Illumina sequencing platform, and then developed 15 polymorphic microsatellite loci in a wild population. A total of 129.46 Gb raw data were obtained, of which 115.07 Gb were clean data, with a sequencing depth of 179.3-folds. This genome was estimated to be 522.6 Mb in size, with the heterozygosity, repeat content and GC content being...
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