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Article

Flow cytometric characterisation of the complex polyploid genome of Saccharum officinarum and modern sugarcane cultivars.

Scientific reports - 18 Dec 2019

Metcalfe Cushla J, Li Jingchuan, Giorgi Debora, Doležel Jaroslav, Piperidis Nathalie, Aitken Karen S

Abstract excerpt

Sugarcane (Saccharum spp.) is a globally important crop for sugar and bioenergy production. Its highly polyploid, complex genome has hindered progress in understanding its molecular structure. Flow cytometric sorting and analysis has been used in other important crops with large genomes to dissect the genome into component chromosomes. Here we present for the first time a method to prepare suspensions of intact...

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PMID: 31852940
DOI: 10.1038/s41598-019-55652-3
PMCID: PMC6920420

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