The development and use of Actiphage ^® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease‐infected animals

Abstract excerpt

Summary Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from low numbers of mycobacterial cells. This method (Actiphage ® ) used in combination with PCR achieved rapid and sensitive (LOD ≤ 10 cell ml −1 ) detection and identification of viable, pathogenic mycobacteria in blood samples within 6 h. We demonstrate that mycobacteriophage D29...

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