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A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy

25 Nov 2019

Abstract excerpt

Contamination of cell cultures by mycoplasmas is a very common phenomenon. As they can substantially alter cell metabolism and potentially spread to all cell cultures in laboratory, their early detection is necessary. One of the fastest and cheapest methods of mycoplasma detection relies on the direct staining of mycoplasmas' DNA by DAPI or Hoechst dyes. Although this method is easy and fast to perform, it...

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PMID: 31775352
DOI: 10.3390/cells8121510

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