Quantitative real time polymerase chain reaction (q RT‐PCR ) and RNA scopein situhybridization ( RNA ‐ ISH ) as effective tools to diagnose feline herpesvirus‐1‐associated dermatitis

Abstract excerpt

Background Felid herpesvirus type 1 (FHV‐1)‐associated dermatitis is characterized by facial and nasal involvement; clinical and histopathological manifestations may overlap with other dermatitides. Objective To evaluate the realibility of qRT‐PCR‐2−ΔΔCqandRNAscopein situhybridization (RNA‐ISH) methods to diagnoseFHV‐1‐associated dermatitis, in formalin‐fixed paraffin‐embedded (FFPE) tissues. Animals...

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