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Effectiveness of 16S ribosomal DNA real-time PCR and sequencing for diagnosing bacterial keratitis

13 Aug 2019

Abstract excerpt

PURPOSE: To evaluate the efficacy of real-time PCR for 16S ribosomal DNA (16S r-DNA) and sequencing for diagnosing microbial keratitis. METHODS: We studied 272 eyes of 272 patients with keratitis. Eyes with keratitis were classified as "definite" (N = 118), "likely" (N = 71), or "non-bacterial" (N = 83) to have bacterial keratitis. The diagnostic efficacy of real-time PCR and conventional testing was determined...

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PMID: 31410557
DOI: 10.1007/s00417-019-04434-8

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