Complementary Roles for Single-Nucleus and Single-Cell RNA Sequencing in Kidney Disease Research

Abstract excerpt

We congratulate Wu et al.1 on their successful application of single-nucleus RNA sequencing to characterize renal cell types underrepresented by single-cell RNA sequencing. A particular strength of their workflow is the detection of cells such as podocytes, mesangial cells, and glomerular endothelial cells, which can be underrepresented by conventional single-cell sequencing. These cell types have previously been...

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