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Development of a Digital Droplet Polymerase Chain Reaction (ddPCR) assay to detect Leishmania DNA in samples from Cutaneous Leishmaniasis patients

3 Nov 2018

Abstract excerpt

AIM: Here, we evaluate the ddPCR platform using an evaluated qPCR-based diagnostic assay for the detection of Leishmania infection in Cutaneous Leishmaniasis patients. METHODS: A standard curve of cultured Leishmania parasite material and clinical samples of CL patients were tested with ddPCR to determine the analytical and diagnostic performance. RESULTS: The limit of detection of the assay on the ddPCR platform...

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PMID: 30399446
DOI: 10.1016/j.ijid.2018.10.029

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