Article
Development of a TaqMan MGB RT-PCR assay for the detection of type A and subtype H10 avian influenza viruses.
Archives of virology - 1 Sept 2018
Yang Fan, Chen Bin, Liu Fumin, Peng Xiuming, Sun Tao, Yao Hangping, Wu Haibo, Wu Nanping
Abstract excerpt
H10 subtype avian influenza viruses have caused several epidemics in poultry and mammals, and specific, rapid and sensitive methods for detection are urgently needed. Herein, TaqMan minor groove binder (MGB) probes and multiplex real-time RT-PCR primers were designed based on gene regions encoding conserved domains of the nucleoprotein and H10 hemagglutinin. The developed multiplex real-time RT-PCR assay...
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