Article
Autofluorescence microscopy for paired-matched morphological and molecular identification of individual chigger mites (Acari: Trombiculidae), the vectors of scrub typhus.
PloS one - 1 Jan 2018
Kumlert Rawadee, Chaisiri Kittipong, Anantatat Tippawan, Stekolnikov Alexandr A, Morand Serge, Prasartvit Anchana, Makepeace Benjamin L, Sungvornyothin Sungsit, Paris Daniel H
Abstract excerpt
BACKGROUND: Conventional gold standard characterization of chigger mites involves chemical preparation procedures (i.e. specimen clearing) for visualization of morphological features, which however contributes to destruction of the arthropod host DNA and any endosymbiont or pathogen DNA harbored within the specimen. METHODOLOGY/PRINCIPAL FINDINGS: In this study, a novel work flow based on autofluorescence...
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