Molecular typing of Legionella pneumophila isolates from environmental water samples and clinical samples using a five-gene sequence typing and standard Sequence-Based Typing.

Abstract excerpt

Inadequate discriminatory power to distinguish between L. pneumophila isolates, especially those belonging to disease-related prevalent sequence types (STs) such as ST1, ST36 and ST47, is an issue of SBT scheme. In this study, we developed a multilocus sequence typing (MLST) scheme based on two non-virulence loci (trpA, cca) and three virulence loci (icmK, lspE, lssD), to genotype 110 L. pneumophila isolates from...

Topics

• China
• Genes, Bacterial
• Genetics, Population
• Genotype
• Humans
• Legionella pneumophila
• Legionnaires' Disease
• Multilocus Sequence Typing
• Phylogeny
• Sequence Analysis, DNA
• Virulence