Article
Human IgG lacking effector functions demonstrate lower FcRn-binding and reduced transplacental transport.
Molecular immunology - 1 Mar 2018
Stapleton Nigel M, Armstrong-Fisher Sylvia S, Andersen Jan Terje, van der Schoot C Ellen, Porter Charlene, Page Kenneth R, Falconer Donald, de Haas Masja, Williamson Lorna M, Clark Michael R, Vidarsson Gestur, Armour Kathryn L
Abstract excerpt
We have previously generated human IgG1 antibodies that were engineered for reduced binding to the classical Fcγ receptors (FcγRI-III) and C1q, thereby eliminating their destructive effector functions (constant region G1Δnab). In their potential use as blocking agents, favorable binding to the neonatal Fc receptor (FcRn) is important to preserve the long half-life typical of IgG. An ability to cross the placenta,...
Topics
- Cells, Cultured
- Female
- Histocompatibility Antigens Class I
- Human Umbilical Vein Endothelial Cells
- Humans
- Immunoglobulin G
- Kinetics
- Maternal-Fetal Exchange
- Models, Molecular
- Mutant Proteins
- Mutation
- Placenta