Article
Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Morel Arry, Peeters Nemo, Vailleau Fabienne, Barberis Patrick, Jiang Gaofei, Berthomé Richard, Guidot Alice
Abstract excerpt
In this chapter, we describe different methods for phenotyping strains or mutants of the bacterial wilt agent, Ralstonia solanacearum, on four different host plants: Arabidopsis thaliana, tomato (Solanum lycopersicum), tobacco (Nicotiana benthamiana), or Medicago truncatula. Methods for preparation of high volume or low volume inocula are first described. Then, we describe the procedures for inoculation of plants...
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