Article
A multiple near isogenic line (multi-NIL) RNA-seq approach to identify candidate genes underpinning QTL.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Mar 2018
Habib Ahsan, Powell Jonathan J, Stiller Jiri, Liu Miao, Shabala Sergey, Zhou Meixue, Gardiner Donald M, Liu Chunji
Abstract excerpt
KEY MESSAGE: This study demonstrates how identification of genes underpinning disease-resistance QTL based on differential expression and SNPs can be improved by performing transcriptomic analysis on multiple near isogenic lines. Transcriptomic analysis has been widely used to understand the genetic basis of a trait of interest by comparing genotypes with contrasting phenotypes. However, these approaches identify...
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