Article
Use of a bioinformatic-assisted primer design strategy to establish a new nested PCR-based method for Cryptosporidium.
Parasites & vectors - 23 Oct 2017
Koehler Anson V, Korhonen Pasi K, Hall Ross S, Young Neil D, Wang Tao, Haydon Shane R, Gasser Robin B
Abstract excerpt
BACKGROUND: The accurate tracking of Cryptosporidium in faecal, water and/or soil samples in water catchment areas is central to developing strategies to manage the potential risk of cryptosporidiosis transmission to humans. Various PCR assays are used for this purpose. Although some assays achieve specific amplification from Cryptosporidium DNA in animal faecal samples, some do not. Indeed, we have observed...
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