Article
Typing and comparative genome analysis of Brucella melitensis isolated from Lebanon.
FEMS microbiology letters - 16 Oct 2017
Abou Zaki Natalia, Salloum Tamara, Osman Marwan, Rafei Rayane, Hamze Monzer, Tokajian Sima
Abstract excerpt
Brucella melitensis is the main causative agent of the zoonotic disease brucellosis. This study aimed at typing and characterizing genetic variation in 33 Brucella isolates recovered from patients in Lebanon. Bruce-ladder multiplex PCR and PCR-RFLP of omp31, omp2a and omp2b were performed. Sixteen representative isolates were chosen for draft-genome sequencing and analyzed to determine variations in virulence,...
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