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Estimation of Human Norovirus Infectivity from Environmental Water Samples by In Situ Capture RT-qPCR Method.

Food and environmental virology - 1 Mar 2018

Tian Peng, Yang David, Shan Lei, Li Qianqian, Liu Danlei, Wang Dapeng

Abstract excerpt

Human noroviruses (HuNoVs) are highly infectious viruses for which water is an important medium of transmission. In this study, we explored a new in situ capture RT-qPCR (ISC-RT-qPCR) methodology to estimate the infectivity of HuNoV in environmental water samples. This assay was based on capturing encapsidated HuNoV by viral receptors, followed by in situ amplification of the captured viral genomes by RT-qPCR. We...

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PMID: 28856596
DOI: 10.1007/s12560-017-9317-1

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