Article
Ribosomal RNA gene probes to detect intraspecies heterogeneity in Mycoplasma gallisepticum and M. synoviae.
Avian diseases - 1 Jan 2000
Yogev D, Levisohn S, Kleven S H, Halachmi D, Razin S
Abstract excerpt
Intraspecies genotypic heterogeneity among strains of Mycoplasma gallisepticum and M. synoviae was tested using genomic fingerprints with a ribosomal RNA (rRNA) gene probe. The organism's DNA was digested by a restriction endonuclease, electrophoresed, transferred to a nitrocellulose sheet, and hybridized with 32P-labeled pMC5 plasmid carrying the highly conserved rRNA genes of M. capricolum. The resulting...
Topics
- Animals
- Bacterial Proteins
- DNA Restriction Enzymes
- DNA, Bacterial
- Deoxyribonuclease EcoRI
- Deoxyribonuclease HindIII
- Deoxyribonucleases, Type II Site-Specific
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Genotype