Article
Evaluation of a hypervariable octameric oligonucleotide fingerprints assay for identification of and discrimination between wild-type and vaccine strains of Brucella melitensis.
American journal of veterinary research - 1 Apr 2017
Tang Lijuan, Liu Jun, Wang Yuanzhi, Zhang Hui, Chen Chuangfu
Abstract excerpt
OBJECTIVE To evaluate a hypervariable octameric oligonucleotide fingerprints (HOOF-Prints) assay for identification of and discrimination between wild-type and vaccine strains of Brucella melitensis. SAMPLE Brucella melitensis vaccine strain M5 and wild-type strain M43. PROCEDURES 8 pairs of primers (alterable, octameric nucleotides) were designed on the basis of a biological analysis of 8 flanking sequences in...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.